ABSTRACT
Objective To explore the diagnostic value of k ras mutation detection of specimen obtained by endoscopic ultrasound-guided fine needle aspiration (EUS-FNA)for pancreatic cancer.Methods Seventy-eight patients with pancreatic carcinoma and 49 patients with pancreatic benign diseases were collected from January 2013 to December 2015.All the patients underwent EUS-FNA and cell or tissue samples were collected.DNA was extracted from the samples,and the codon 12 and 13 mutation of K-ras gene was detected by specific nano capture probe.Liquid cytology was also conducted.The sensitivity and specificity of the two methods were compared.Results The K-ras mutation rate was 92.3％ (72/78) in 78 patients with pancreatic carcinoma,which was obviously higher than the mutation rate of 20.4％ (10/49) in 49 patients with pancreatic benign tumors,and the difference was statistically significant (x2 =68.002,P =0.000).The sensitivity of the cytology examination of EUS-FNA specimens in the diagnosis of pancreatic carcinoma was 75.6％,the specificity was 87.8％,Youden index was 0.634,the positive and negative predictive value was 6.196 and 0.227.The detection of K-ras mutations had a sensitivity of 92.3％ and a specificity of 79.6％ in the diagnosis of pancreatic carcinoma,the Youden index was 0.719,the positive and negative predictive value was 4.524 and 0.096.K-ras mutation detection had a higher sensitivity compared with cytology,and the difference was statistically significant (x2 =8.47,P =0.004).The sensitivity was 94.9％ and specificity of 95.9％ using the combination of K-ras mutations and cytology for diagnosing pancreatic cancer,and the specificity was obviously increased compared with only k ras mutation detection and the difference was statistically significant (x2 =6.13,P =0.013).Conclusions K-ras mutation detection of EUS-FNA specimen using nano capture probe system can significantly improve the sensitivity of diagnosing pancreatic cancer,and the sensitivity and specificity could be further improved when combined with cytology.
ABSTRACT
Objective To evaluate the clinical value of three-step technology for screening of cervical lesions .Methods From March 2011 to March 2013 , 6965 outpatients and inpatients were detected with the ThinPrep liquid based cytology test ( TCT) in our hospital , at the same time, cytology positive cases were evaluated with colposcopic multiple biopsies and histopathology .Results Three hundred and thirty -four cases were positive in cytology, in which there were 187 cases of ASCUS(55.99%),40 cases of ASC-H(11.98%),48 cases of LSIL(14.37%),52 cases of HSIL(15.57%) and 7 cases of SCC(2.10%).Among the 286 cases with cy-tological abnormalities diagnosed with histopathology , 97 cases with CINI(33.92%),85 cases with CINII/CINIII(29.72%)2,0 cases with SCC(6.99%),and the positive coincidence rate of colposcopy was 98.06%.Histopathology proved to 57 CINI, 127 CINII/CINI-II and 22 SCC, so the total positive rate of cervical lesions was 2.96%.Conclusions Three-step technique in combination application of cytology , colposcopic biopsy and histopathology can be an effective screening of cervical lesions .